In-vitro probiotic properties of Lactobacillus satsumensis LC311746.1 isolated from water kefir grains

Abstract

Lactobacillus satsumensis LC311746.1 was isolated from water kefir grains. Then, the bacterial isolate was tested for gastrointestinal conditions, hydrophobicity, aggregation ability, antibiotic resistance, antimicrobial properties, and cholesterol-lowering properties. The results showed that the bacterial isolate was resistant to acidic conditions even after 3 hours of exposure. Resistance to bile salt, pepsin, and pancreatin enzymes was also greater than 90% at the end of each experiment. The isolate had a relatively low hydrophobic capacity of up to 20%, but had a high autoaggregation capacity. On the other hand, the isolate had relatively low coaggregation ability with Staphylococcus aureus. In addition, the isolate was sensitive to more than two antibiotics, indicating that it was safe for human consumption. Also, L. satsumensis showed medium antimicrobial activities against pathogenic bacteria, e.g. Campylobacter jejuni, Escherichia coli, Salmonella Enteritidis, Listeria monocytogenes and Salmonella Typhimurium. The cholesterol reducing ability of the isolate was also detected. In conclusion, L. satsumensis LC311746.1 has promising probiotic properties that could be useful for the development of novel health-friendly food products.