Visualization of interaction between Pochonia Chlamydosporia and Meloidogyne incognita and their impact on tomato crop

Authors

  • Wajid Khan Centre for Biotechnology and Microbiology University of Swat, Khyber Pakhtunkhwa
  • Saifullah Department of Plant Pathology, Khyber Pakhtunkhwa Agricultural University Peshawar
  • Sher Hayat Khan Department of Animal Health, Parasitology Section, Khyber Pakhtunkhwa Agricultural University Peshawar
  • Musharaf Ahmad Department of Plant Pathology, Khyber Pakhtunkhwa Agricultural University Peshawar
  • Aqib Iqbal Institute of Biotechnology and Genetic Engineering, Khyber Pakhtunkhwa Agricultural University Peshawar
  • Wajid Khan Centre for Biotechnology and Microbiology University of Swat, Khyber Pakhtunkhwa

Abstract

In present studies, the biocontrol potential of Pochonia Chlamydosporia was evaluated against Meloidogyne incognita. In invitro condition, P. chlamydosporia was tested for parasitism of eggs and females of M. incognita. The result indicated that this fungus parasitized 87% eggs and 82% of females. The culture filtrate (CF) of P. chlamydosporia was tested for its larvicide activity at four different concentrations i.e., 25, 50, 75 and 100 % against M. incognita 2nd stage juvenile. The mortality of 2nd stage juvenile was directly proportioned to the concentration of the CF and to the duration of exposure. The maximum mortality was 97.3% at 100% concentration of the culture filtrate after 96 hours followed by 86.6% at 75% concentration. While minimum mortality was 7.3% in 25% concentration after 24 hrs. The result of the pot experiment proved that P. chlamydosporia has reduced the incidence of RKN and improved all tested agronomic growth parameters.  The treatment with inoculated M. incognita alone reduced plant height, fresh shoot, and fresh root weight by 44.7%, 29.8%, and 32.8% respectively over uninoculated healthy control. P. chlamydosporia improved all the tested growth parameters in presence of M. incognita. Histopathological studies on the interaction of Pochonia chlamydosporia and Meloidogyne incognita on tomato roots revealed anatomical changes among treatments. Less number of galls with tiny in size and scarcer abnormalities in the vascular cylinder was observed in plants inoculated with P. chlamydosporia and M. incognita than the plants treated with nematode only. The fungus was seen in in the intercellular spaces of cortical and epidermal cells while the vascular bundles of the plant remain intact, inoculated only with P. chlamydosporia. The plants treated with both fungus and M. incognita, P. chlamydosporia was found in the vascular region affected by the nematode. In the infected roots, many mature females were seen which feed on giant cells. The findings also revealed that control healthy plants were not affected and no histological changes were noted.

Key words: Histopathology, Pochonia chlamydosporia, Meloidogyne incognita, tomato

Published

10-10-2023

How to Cite

Khan, W., Saifullah, Khan, S. H., Ahmad, M., Iqbal, A., & Khan, W. (2023). Visualization of interaction between Pochonia Chlamydosporia and Meloidogyne incognita and their impact on tomato crop . 3rd International Congress of the Turkish Journal of Agriculture - Food Science and Technology, Malatya, Türkiye, 442–442. from http://turjaf.com/index.php/TURSTEP/article/view/264