RNAi-Mediated Post-Transcriptional Gene Silencing Strategies in Honey Bees

Abstract

Gene silencing technology (knock-down) via RNA interference (RNAi), a contemporary molecular method, represents an RNA-based, sequence-specific, post-transcriptional approach. RNAi has demonstrated its efficacy in reducing gene expression across a wide array of organisms, encompassing plants, mammals, and insects. Studies conducted on diverse organism groups have significantly contributed to comprehending both the operational mechanism of RNAi and the roles of genes within these organisms' biological processes. Among the organisms benefiting extensively from RNAi is the honey bee Apis mellifera. This technology, serving as a crucial natural antiviral defense mechanism in bees, has proven highly effective in probing genes associated with the immune system and in combatting pests and pathogens. Consequently, this study undertakes a review of the existing literature to delineate the potential applications, usage areas, and strategies of RNAi technology in honey bees.