Antimicrobial And Biogenic Synthesis Of Selenium Nanoparticles By Lactobacillus Casei

Abstract

Lactic acid bacteria have long been consumed by people in several fermented foods such as dairy products. Selenium has a great potential such as supporting immune system, fertility, antimicrobial and anticancer agent. This study is aimed at characterization, and investigation of the antimicrobial activity of Selenium nanoparticles synthesized by Lactobacillus casei. Selenium nanoparticles was biosynthesized using L. casei and was characterized using Visual detection, UV-Visible spectrophotometry, Fourier Transform Infra-red spectrophotometry (FTIR), Scanning Electron Microscope (SEM), Transmission Electron Microscope (TEM), Energy Dispersive X-ray (EDX) and X-ray Diffraction (XRD) analysis. The antimicrobial activity of the synthesized selenium nanoparticles using Lactobacillus casei (LC-SeNPs) was done using agar well diffusion method. There was a change in colour from light yellow to ruby red. The broad band for LC-SeNPs ranged from 250 - 450 nm and a strong plasmon resonance peak was at 350 nm. Hydroxyl, esters, aldehyde, amine, phenol, and alkyl aryl, ether are present and responsible for the efficient stabilization and bio-reduction. The SeNPs were spherical and aggregated with varying shapes and size ranged from 20 – 100 nm. The XRD reveals that the SeNPs was crystallographic. LC-SeNPs exhibited an effective inhibitory activity ranging from 10 – 25 mm. Also, biosynthesized SeNPs by L. casei is ecofriendly, nontoxic and highly applicable in medicine.