OPTIMIZATION OF ULTRASONIC ASSISTED EXTRACTION OF BIOACTIVE COMPOUNDS OF RUMEX ACETOSELLA

Abstract

Aim: This study aims to optimize ultrasonication time, ethanol concentration, and solid-to-solvent ratio for the optimization of the ultrasonic extraction of bioactive compounds from Rumex acetosella. Method: A second-order model for the response variables was developed to obtain quadratic equations. Total phenolic content (TPC) was measured by spectrophotometric absorbance analysis, and the total phenolic compound concentration was calculated as gallic acid equivalent (GAE). Absorbance values were recorded, and calibration curves were generated for analysis. Antioxidant activity (AOA) was evaluated using the DPPH method. The percentage of DPPH radical neutralization was calculated using control and sample absorbance values Results: The quadratic models were significant (p < 0.05) with insignificant lack of fit, demonstrating a good fit, and the coefficient of determination values for TPC and AOA were 98.52 and 97.43, respectively. Under optimum process variables of 10 min., 90% ethanol concentration and 0.36 solid-to-solvent ratio, TPC was 692.92 ± 15.38 mg GAE/g, and AOA was 68.50 ± 12.74%. Conclusion: Findings indicate that ultrasound-assisted extraction is an effective method for optimizing the extraction of bioactive compounds from Rumex acetosella, a high total phenolic content (TPC) and antioxidant activity (AOA) under carefully controlled processing conditions. The optimized parameters demonstrated the potential of this technique to maximize the recovery of phenolic compounds and antioxidant properties, making it a promising approach for the extraction of bioactive compounds in food applications.